lipofectamine相关论文
The Method of Attaining high transfection efficiency of plasmid into neural stem cells by electropor
Objective:To optimize the conditions of transfecting eukaryotic expression vector pEGFP-N1 into neural stem cells(NSCs)f......
目的 研究针对MDR1基因的反义寡核柑酸对卵巢癌耐药细胞株SKOV3/mdr1耐药性的逆转作用。方法 将MDR1基因导入卵巢癌细胞株SKOV3......
Study on Inhibitionof Growth of ACHN Cells via shRNA Expression Vector-Mediated Cycline1 Gene Silenc
OBJECTIVE To explore the inhibition of ACHN cells via shRNAexpression vector mediated cyclinE1 gene silencing.METHODS Th......
目的优化LipofectamineTM2000介导外源基因转染Neuro-2a(N-2a)细胞的条件,以提高转染效率。方法将携带增强型绿色荧光蛋白(Enhance......
Telomerase plays an important role in cell proliferation and carcinogenesis and is believed to be a good target for anti......
Inhibitory effect of endostatin expressed by human liver carcinoma SMMC7721 on endothelial cell prol
AIM:To construct a stable transfectant of human livercarcinoma cell line SMVLC7721 that could secret humanendestatin an......
AIM To study the effect of hepatitis C virus nonstructuralprotein 3 c-terminal deleted protein(HCV NS3-5’)onhepatocyte ......
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Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray
AIM:Recombined plasmid pETNF-P16 was constructed toinvestigate its expression properties in esophagealsquamous carcinom......
Construction and identification of recombinant vectors carrying herpes simplex virus thymidine kinas
AIM:To construct and identify the recombinant vectorscarrying herpes simplex virus thymidine kinase (HSV-TK) andtumor n......
AIM:To study the effect of cyclin G2 on proliferation of gastricadenocarcinoma cell line-SGC-7901 cell in vitro.METHODS......
AIM:To construct an eukaryotic superantigen gene expressionvector containing the recombinant gene of SEA and CD80molecu......
目的 探索WAF1 S基因在喉癌细胞内表达的作用 ,为喉癌的基因治疗提供理论依据。方法 采用亚克隆技术 ,构建WAF1 S真核表达载体......
Expression of TIMP-3 Gene by Construction of a Eukaryotic Cell Expression Vector and Its Role in Red
The present study is aimed at studying the gene for TIMP-3,a mammalian tissue inhibitor,by constructing arecombinant eu......
Liposome transfected to plasmid-encoding endostatin gene combined with radiotherapy inhibits liver c
AIM: To evaluate whether intratumoral injection of liposome-endostatin complexes could enhance the antitumor efficacy of......
Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by re
AIM To observe the tumor inhibitory effects bytransfecting IL-6 cDNA into colon cancer cell lineHT-29 with retroviral v......
目的 :制备具有治疗活性的野生型p5 3基因真核表达载体的细胞疫苗。方法 :利用RT PCR从人外周血淋巴细胞总mRNA中扩增出全长的野生......
Expression of nuclear factor-KB in hepatocellular carcinoma and its relation with the X protein of h
AIM In this study we investigated therelationship of the X protein of HBV and nuclearfactor-κB(NF-κB)and the expressi......
AIM To compare the effects of liposomes andglyco-poly-L-lysine on liver targeted uptake andexpression of plasmid in rat......
Antisense RhoC gene suppresses proliferation and invasion capacity of human QBC939 cholangiocarcinom
BACKGROUND:Cholangiocarcinoma is a highly aggres- sive,fatal malignancy,which is resistant to all current therapeutic ap......
TIMP-3 gene transfection suppresses invasive and metastatic capacity of human hepatocarcinoma cell l
BACKGROUND:Tissue inhibitor of metalloproteinases (TIMPs)can restrain the tumor growth by their protein property and mat......
Construction of eukaryotic expression vector carrying human TSLC1 gene and its expression in HepG2 c
Objective:To construct the eukaryotic expression vector for human TSLC1 gene,and to express TSLC1 in HepG2 cells for inv......
RNA interference-mediated gene silencing of vascular endothelial growth factor in colon cancer cells
AIM:To inhibit the expression of vascular endothelial growth factor(VEGF)in colon cancer cell line by RNA interference(R......
Objective To investigate the influence of hepatitis B virus X gene(HBx)on apoptosis of hepatic cells mediated by Fas in ......
The bioactivities of culture supernatants from retroviral packaging cells carrying the mouse Fas ligand (mFasL) gene was......
Effect of Antisense Oligodeoxynucleotide Directed to NF-κB-RelA on Bcl-x_L mRNA in Extended Drug Res
To explore the effect of NF κB on bcl x gene transcription in extended drug resistance leukemia cell line HL 60/E6, ......
The effect of transforming growth factor β 1 (TGF β 1 ) gene transfection on the proliferation of bone marro......
AIM: To investigate the contribution of HBV in the development of hepatocarcinoma by examining the effects of HBV on p53......
Gene transfer of somatostatin receptor type 2 by intratumoral injection inhibits established pancrea
AIM: To investigate the therapeutic effect of somatostatin receptor type 2 (SSTR2) gene transfection on pancreatic carci......
Effect of hepatitis C virus nonstructural protein NS3 on proliferation and MAPK phosphorylation of n
AIM: To study the effect of hepatitis C virus nonstructural region 3 (HCV NS3) protein on proliferation and transformati......
Labeling of human insulin-like growth factor-I eukaryotic expression vector with green fluorescent p
Objective: To label human insulin-like growth factor-I (hIGF-I) eukaryotic expression vector with green fluorescent prot......
AIM:To investigate the role of overexpression of Bax in apoptotic pathways and the response of human hepatocellular canc......
目的在仓鼠卵巢细胞CHO细胞中高效表达糖基磷脂酰肌醇GPI-B7-1(B7-1即CD80)融合蛋白,获得大量融合蛋白以研究GPI-B7-1在肿瘤治疗中......
To explore the feasibility of stable expression of Hantavirus H8205 strain G1 segment and human IL-2 fusion gene in Vero......
目的:了解G3BP蛋白在细胞中的表达及定位。方法:利用脂质体lipofectamine2000将提取的质粒pEGFP-CI-G3BP转染进入Hela细胞,培养24h......
Suppressive Effects of Genomic Imprinted Gene PEG10 on Hydrogen Peroxide-induced Apoptosis in L0_2 C
The effects of PEG10 on hydrogen peroxide (H2O2)-induced apoptosis in human normal liver cell line L02 were investigated......
背景:自杀基因独有的旁观者效应,可显著提供肿瘤细胞杀伤效果,同时还与放射治疗、免疫基因治疗联合应用,并克服了基因转导效率低的......
本研究通过体外评价比较了不同阳离子脂材(DC-Chol和DOTAP),不同比例PEG修饰的阳离子脂质体对siRNA细胞转染效果的影响。体外细胞......
Objective:To construct and identify recombinant plasmid pUIS3-BLC~+.Methods:The cDNA of B-lymphocyte chemoattractant(BLC......
Objective: The aim of this study was to explore the effects on malignant proliferation of A549 cell by silencing cy-cloo......
Expression of T-STAR gene is associated with regulation of telomerase activity in human colon cancer
AIM: To investigate the effects on telomerase activity of transfection of human T-STAR gene full-length sense cDNA or pa......
Effect of miR-155 on immune-factors and its mechanism in mesenchymal stem cells under hypoxic enviro
Objective To investigate the effect of miR-155 on immune-factors and its mechanism in mesenchymal stem cells under hypox......
Biological effect of expression of exogenous human nuclear receptor hLRH-1 in SW480 cells and its mo
Objective: To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of......
Effect on Survivin Regulation of Transcription Level by p21~(waf1) Overexpression in HepG2 Hepatocel
The effect of cyclin-dependent kinase inhibitors Cip1/Waf1(p21) on regulatory expression of survivin transcription in hu......
Activator protein-1 involved in growth inhibition by RASSF1A gene in the human gastric carcinoma cel
AIM:To investigate the role of Ras association domain family protein 1 isoform A (RASSF1A) in gastric tumorigenesis. MET......
Down-regulation of Tissue Factor by siRNA Increased Doxorubi-cin-induced Apoptosis in Human Neurobla
The effects of tissue factor (TF) on doxorubicin-induced apoptosis in human neuroblas-toma were investigated. The expres......
Objective:We explored the interfering effect of COX-2 gene expression and the influence on the malignant proliferation o......
Objective:To construct eukaryotic expression plasmid pEGFP-N1-WWOX and transiently express it in SMMC-7721 cells.Methods......
Small Interfering RNA Targeting MDR1 Inhibits Ovarian Cancer Growth and Increases Efficacy of Chemot
Objective:To further validate a knockdown approach for circumventing the multidrug resistance gene(MDR1), we used small ......
